Al,
Everything I've heard/read on disk optimizing under OS X seems to point to DO NOTHING!
If you keep up on the daily/weekly/monthly cron scripts and repair permissions when needed, that's all that is needed.
Fragmentation doesn't seem to apply, as much, when you're talking about speed issues.
If you're concerned, you could always use Carbon Copy Cloner to write an optimized disk and do some comparison tests.